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KMID : 0364820160520040487
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Korean Journal of Microbiology
2016 Volume.52 No. 4 p.487 ~ p.494
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Effects of Paf1 complex components on H3K4 methylation in budding yeast
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Oh Jun-Soo
Lee Jung-Shin
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Abstract
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In Saccharomyces cerevisiae, Paf1 complex consists of five proteins, and they are structurally and functionally well
conserved in yeast, fruit fly, plants, and human. With binding to RNA polymerase II from transcription start site to termination site, Paf1 complex functions as a platform for recruiting many types of transcription factors to RNA polymerase II. Paf1 complex contributes to H2B ubiquitination and indirectly influences on H3K4 di- and tri-methylation by histone crosstalk. But the individual effects of five components in Paf1 complex on these two histone modifications including H2B ubiquitination and H3K4 methylation largely remained to be identified. In this study, we constructed the single-gene knockout mutants of each Paf1 complex component and observed H3K4 mono-, di-, and trimethylation as well as H2B ubiquitination in these mutants. Interestingly, in each Δpaf1, Δrtf1, and Δctr9 strain, we observed the dramatic defect in H3K4 monomethylation, which is independent of H2B ubiquitination, as well as H3K4 di- and trimethylation. However,
the protein level of Set1, which is methyltransferase for H3K4, was not changed in these mutants. This suggests that Paf1 complex may directly influence on H3K4 methylation by directly regulating the activity of Set1 or the stability of Set1 complex in an H2B ubiquitination independent manner.
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KEYWORD
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H3K4 methylation, Paf1 complex, Set1
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